Translated[Free Community Edition] [Enterprise Edition] Webfunny is a big data analysis system that combines a full-link monitoring system and a user behavior analysis (event tracking) system. We are committed to solving difficult problems in production and to fine-grained analysis of business data. The monitoring system is aimed at technology and the event tracking system is aimed at business; used together, they complement each other well.
- web-monitor
- monitoring
- monitoring-tool
- frontend-monitoring
Agent-ready RPA suite with out-of-the-box automation tools. Built for individuals and enterprises.
A one page , two asymmetric column resume template in XeTeX that caters to an undergraduate Computer Science student
It is my belief that you, the postgraduate students and job-seekers for whom the book is primarily meant will benefit from reading it; however, it is my hope that even the most experienced researchers will find it fascinating as well.
Practice on cifar100(ResNet, DenseNet, VGG, GoogleNet, InceptionV3, InceptionV4, Inception-ResNetv2, Xception, Resnet In Resnet, ResNext,ShuffleNet, ShuffleNetv2, MobileNet, MobileNetv2, SqueezeNet, NasNet, Residual Attention Network, SENet, WideResNet)
Browser extension to curate, annotate, and discuss the most valuable content and ideas on the web. As individuals, teams and communities.
- web-extension
- chrome-extension
- firefox-addon
- typescript
The media player for language learning, with dual subtitles, AI-generated subtitles, real-time translation, and more!
Feature-rich wifi hotspot creator for Linux which provides both GUI and command-line interface. It is also able to create a hotspot using the same wifi card which is connected to an AP already ( Similar to Windows 10).
业界首个支持渐进式组件化改造的Android组件化开源框架，支持跨进程调用。Componentize your android project gradually.
- cc
- android-architecture
- android-component
- component
Zhejiang University Graduation Thesis LaTeX Template
- latex
- zjuthesis
- latex-template
- latex-thesis-template
Enhanced MCP server for interactive user feedback and command execution in AI-assisted development, featuring dual interface support (Web UI and Desktop Application) with intelligent environment detection and cross-platform compatibility.
TranslatedHongguo Short Drama PC version · No Android emulator needed, watch Hongguo short dramas directly on Windows / macOS · Download in China: hongguo.235698.xyz · If it won't open, use Lanzou Cloud (no password needed): wwalp.lanzout.com/b0rbj88ji
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Claude Code plugin that generates individualized knowledge systems from conversation. You describe how you think and work, have a conversation and get a complete second brain as markdown files you own.
Auto-register & manage accounts for ChatGPT, Cursor, Kiro, Grok, Windsurf, Trae & 13+ AI platforms · Protocol/browser dual-mode · Plugin-based · One-click Mac/Windows desktop app
- account-management
- account-registration
- ai
- ai-tools
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
:racehorse:基于SpringBoot + MySQL + Redis + RabbitMQ + Guava开发的高并发商品限时秒杀系统
mrdoc,online document system developed based on python. It is suitable for individuals and small teams to manage documents, wiki, knowledge and notes. 觅思文档，适合于个人和中小型团队的在线文档、AI知识库系统。
A python application that detects and highlights the heart-rate of an individual (using only their own webcam) in real-time.
Font Awesome split to individual SVG and PNG files of different sizes along with Node.JS based generator
GPU Miner for ETH, RVN, BEAM, CFX, ZIL, AE, ERGO
- ethminer
- mining
- dual-mining
- eth
Fast, portable, non-Turing complete expression evaluation with gradual typing (Go)
Turn your Pico 2 W into a DualSense 5 dongle.
中国科研常用LaTeX模板集