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ShareX is a free and open-source application that enables users to capture or record any area of their screen with a single keystroke. It also supports uploading images, text, and various file types to a wide range of destinations.
Ip2region is an offline IP-to-Region localization library and IP data management framework with both IPv4 and IPv6 supports, 10-microsecond level query efficiency, xdb search client for many programming languages
Capture Screen, Audio, Cursor, Mouse Clicks and Keystrokes
YugabyteDB - the cloud native distributed SQL database for mission-critical applications.
🌍 Discover our global repository of countries, states, and cities! 🏙️ Get comprehensive data in JSON, SQL, PSQL, SQLSERVER, MONGODB, SQLITE, XML, YAML, and CSV formats. Access ISO2, ISO3 codes, country code, capital, native language, timezones (for countries), and more. #countries #states #cities
An MobileGestalt editor for iOS 27.x, featuring region-code tweaks, device presets, backups.
Restore videos with pixelated/mosaic regions
A bash script to check the status of your IP on various geo-restricted services.
A tool to select chunks from Minecraft worlds for deletion or export.
Fork of Paper which adds regionised multithreading to the dedicated server.
A large scale non-linear optimization library
PyTorch implementation of Advantage Actor Critic (A2C), Proximal Policy Optimization (PPO), Scalable trust-region method for deep reinforcement learning using Kronecker-factored approximation (ACKTR) and Generative Adversarial Imitation Learning (GAIL).
List for blocking ads in the Chinese region. Block Ads, Trackers, Malware, HTTPDNS, PCDN.
Official implementation of Character Region Awareness for Text Detection (CRAFT)
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
最全最新中国省，市，地区json及sql数据
提升部署在cloudflare、vercel或netlify的网页在中国的访问速度和稳定性 cf优选域名 | cf优选ip | cloudflare | vercel | netlify | 加速 | 国内 | 中国 | 境内 | 大陆
Learn step-by-step how to scrape Google Trends data and make a result comparison using Python and Oxylabs SERP API. Extract keywords, their popularity, breakdown by region, related queries, and more.
BRPickerView 封装的是iOS中常用的选择器组件，主要包括：日期选择器（支持年月日、年月等15种日期样式选择，支持设置星期、至今等）、地址选择器（支持省市区、省市、省三种地区选择）、自定义字符串选择器（支持单列、多列、二级联动、三级联动选择）。支持自定义主题样式，适配深色模式，支持将选择器组件添加到指定容器视图。
ISO 3166-1 country lists merged with their UN Geoscheme regional codes in ready-to-use JSON, XML, CSV data sets
R-CNN: Regions with Convolutional Neural Network Features
TranslatedA self-hosted address and synthetic test-profile generator for 27 countries and regions, built from real open-data streets, administrative areas, coordinates, and postcodes. Supports multilingual output, IP-nearby generation, map previews, and API access.
maim (make image) takes screenshots of your desktop. It has options to take only a region, and relies on slop to query for regions. maim is supposed to be an improved scrot.
Spring Cloud 微服务开发核心工具集。工具类、验证码、http、redis、ip2region、xss 等，开箱即用。 🔝 🔝 记得右上角点个star 关注更新！